FLOW CYTOMETER 

Pharmidex's Cytometry service covers nearly everything from basic immunology, cell health, functional assays, drug screening, host–pathogen interactions, extracellular vesicles and high-throughput pharmacology, except cell sorting, which it cannot perform.

High-throughput Screening

96- or 384-well plate–assays

• Drug cytotoxicity screens.
• Immunomodulator assays (checkpoint inhibitors, cytokine inducers).
• Small molecule or siRNA screens.

Kinetics & dose-response

in multi-well format.

Outputs

Plate heatmaps, IC50/EC50 calculations, multiparametric readouts per well.

Microbiology & Host–Pathogen Studies

Bacterial counts & viability 

SYTO dyes, PI.

Fungal cell profiling 

yeast budding, hyphal growth.

Viral infection assays 

reporter gene expression, antigen staining.

Host–pathogen interaction 

bacteria/parasite uptake by immune cells.



Outputs

% infected cells, pathogen load, immune response markers.

Immunophenotyping

Leukocyte subsetting 

T cells (CD4, CD8), B cells, NK cells, monocytes, granulocytes.

Lineage panels

Hematopoietic stem/progenitor cells, myeloid vs lymphoid profiling.

BALF/lung immune profiling macrophages, neutrophils, eosinophils, ILCs.

T cell subsets

Th1/Th2/Th17/Th9, Tregs.

Outputs 

% of populations, median fluorescence intensity, 

co-expression patterns.

Functional Immune Assays

Cytokine production

intracellular staining (IFN-γ, IL-4, TNF-α, IL-17, etc).

Degranulation

CD107a mobilization.

Phagocytosis assays

FITC-labeled bacteria/beads uptake.

Oxidative burst assays 

dihydrorhodamine, luminol-based.

Proliferation tracking

CFSE, CellTrace Violet, eFluor

Outputs

% cytokine-positive cells, MFI shifts, proliferation index, phagocytic activity.

Extracellular Vesicles & Microparticles

Exosome/microvesicle detection

size <1 µm, fluorescence labeling.

Bead-based capture assays

EV surface proteins, multiplex exosome markers.



Outputs

EV concentration (events/µL), marker positivity, relative distribution.

Cell Health & Viability

Live/dead discrimination

Zombie dyes, PI, 7-AAD, DAPI.

Apoptosis/necrosis assays Annexin V + PI.

Mitochondrial health

JC-1, TMRE/TMRM (Δψm).

ROS detection

DCFDA, CellROX.

Cell cycle analysis

DNA content (PI, DAPI, Hoechst).

Outputs

% viable/apoptotic/necrotic cells, ROS levels, mitochondrial depolarization, G0/G1/S/G2/M phase fractions.

Cellular Therapeutics

CAR-T characterization

CAR expression, T-cell phenotype, exhaustion markers.

CRISPR/Transfection

GFP/mCherry reporters, knock-in validation.

Drug screening

cytotoxicity, apoptosis induction.

Cell line authentication

marker expression panels.



Outputs

% transduced cells, dose-response curves, biomarker validation.

Rare Event Detection

Minimal residual disease  monitoring in hematology.

Circulating tumor cells (CTCs) detection with antibody panels.

Stem cell frequency analysis LSK, CD34+, etc.



Outputs

Rare-event frequencies, gating strategies for clinical-like detection.

Bead-based Multiplexing Assays

Cytokine bead arrays

CBA, LEGENDplex, Luminex-type on flow.

Immunoassays:

antigen/antibody quantification.

Standardization & QC beads

fluorescence calibration, compensation.

Outputs

Concentration curves, cytokine quantification (pg/mL), calibration QC.

Specialized Applications

Calcium flux assays

Fluo-4, Indo-1.

Membrane potential

DiBAC, voltage-sensitive dyes.

Adhesion/migration studies

integrins, chemotaxis readouts.

Plant & algae cytometry

chlorophyll autofluorescence.

Nanoparticle uptake/toxicity

fluorescent nanomaterials.

Outputs

Dynamic fluorescence changes, uptake efficiency, stress responses.

Outputs and Data Types

Absolute counts

with beads or volumetric.

Relative % populations  immunophenotyping.

Fluorescence intensity shifts (MFI).

Proliferation/division indices.

Kinetic curves

Ca²⁺ flux, ROS, drug effects.

High-throughput data tables

plate-based assays.



Rare-event frequencies.