FLOW CYTOMETRY
Attune NxT Acoustic Flow Cytometer + CytKick Max Autosampler is a flexible benchtop cytometer with acoustic focusing,
12 fluorescence channels and high-throughput plate handling. It can perform a huge range of cell and particle-based assays.
Pharmidex's Cytometry service covers nearly everything from basic immunology, cell health, functional assays, drug screening, host–pathogen interactions, extracellular vesicles and high-throughput pharmacology.
Outputs and Data Types
Absolute counts with beads or volumetric.
Relative % populations immunophenotyping.
Fluorescence intensity shifts (MFI)
Proliferation/division indices
Kinetic curves Ca²⁺ flux, ROS, drug effects.
High-throughput data tables plate-based assays.
Rare-event frequencies
Immunophenotyping
Leukocyte subsetting
T cells (CD4, CD8), B cells, NK cells, monocytes, granulocytes.
Lineage panels
Hematopoietic stem/progenitor cells, myeloid vs lymphoid profiling.
BALF/lung immune profiling Macrophages, neutrophils, eosinophils, ILCs.
T cell subsets
Th1/Th2/Th17/Th9, Tregs.
Outputs
% of populations, median fluorescence intensity,
co-expression patterns.
Cell Health & Viability
Live/dead discrimination
Zombie dyes, PI, 7-AAD, DAPI.
Apoptosis/necrosis assays
Annexin V + PI.
Mitochondrial health:
JC-1, TMRE/TMRM (Δψm).
ROS detection:
DCFDA, CellROX.
Cell cycle analysis:
DNA content (PI, DAPI, Hoechst).
Outputs
% viable/apoptotic/necrotic cells, ROS levels, mitochondrial depolarization, G0/G1/S/G2/M phase fractions.
Functional Immune Assays
Cytokine production
Intracellular staining (IFN-γ, IL-4, TNF-α, IL-17)
Degranulation
CD107a mobilization.
Phagocytosis assays
FITC-labeled bacteria/beads uptake.
Oxidative burst assays
Dihydrorhodamine, luminol-based.
Proliferation tracking
CFSE, CellTrace Violet, eFluor)
Outputs
% cytokine-positive cells, MFI shifts, proliferation index, phagocytic activity.
Cellular Therapeutics
CAR-T characterization
CAR expression, T-cell phenotype, exhaustion markers.
CRISPR/Transfection
GFP/mCherry reporters, knock-in validation.
Drug screening
Cytotoxicity, apoptosis induction.
Cell line authentication
marker expression panels.
Outputs
% transduced cells, dose-response curves, biomarker validation.
High-throughput Screening
96- or 384-well plate–assays:
- Drug cytotoxicity screens.
- Immunomodulator assays (checkpoint inhibitors, cytokine inducers).
- Small molecule or siRNA screens.
Kinetics & dose-response
In multi-well format.
Outputs
Plate heatmaps, IC50/EC50 calculations, multiparametric readouts per well.
Microbiology & Host–Pathogen Studies
Bacterial counts & viability
SYTO dyes, PI.
Fungal cell profiling
Yeast budding, hyphal growth.
Viral infection assays
Reporter gene expression, antigen staining.
Host–pathogen interaction
Bacteria/parasite uptake by immune cells.
Outputs
% infected cells, pathogen load, immune response markers.
Extracellular Vesicles & Microparticles
Exosome/microvesicle detection
Size <1 µm, fluorescence labeling.
Bead-based capture assays
EV surface proteins, multiplex exosome markers.
Outputs
EV concentration (events/µL), marker positivity, relative distribution.
Bead-based Multiplexing Assays
Cytokine bead arrays
CBA, LEGENDplex, Luminex-type on flow.
Immunoassays:
Antigen/antibody quantification.
Standardization & QC beads
Fluorescence calibration, compensation.
Outputs
Concentration curves, cytokine quantification (pg/mL), calibration QC.
Specialized Applications
Calcium flux assays
Fluo-4, Indo-1.
Membrane potential
DiBAC, voltage-sensitive dyes.
Adhesion/migration studies
Integrins, chemotaxis readouts.
Plant & algae cytometry
Chlorophyll autofluorescence.
Nanoparticle uptake/toxicity
Fluorescent nanomaterials.
Outputs
Dynamic fluorescence changes, uptake efficiency, stress responses.
Rare Event Detection
Minimal residual disease
Monitoring in hematology.
Circulating tumor cells (CTCs)
Detection with antibody panels.
Stem cell frequency analysis
LSK, CD34+, etc.
Outputs
Rare-event frequencies (as low as 1 in 10^6), gating strategies for clinical-like detection.
