Flow Cytometry Services

• Analytical Excellence

Why Powered by Pharmidex

Outputs & Data Deliverables

Integrating this capability with our services opens up exciting synergies:

• Histology & imaging: correlate cell subsets with tissue architecture

• Biomarker analysis: cross-validate flow-based biomarker readouts with proteomics, ELISA, transcriptomics

• In vivo & disease models: combine flow readouts from tissues/fluids with in vivo pharmacology, toxicology, and PK/PD

• Autoimmune & immuno-oncology studies: dissect immune cell phenotypes, function, and kinetics in preclinical models

• Absolute counts of subsets

• Relative population percentages (immunophenotyping)

• Mean fluorescence intensity shifts (MFI),

• Proliferation and division indices

• Kinetic curves (Ca²⁺ flux, ROS, drug effects.)

• High-throughput data tables (plate-based assays)

• Rare event frequencies (for rigorous study documentation)

+ Assay Portfolio

Core Cytometry Services

Immunophenotyping

Cell Health & Viability

• Leukocyte subsetting including CD4, CD8, B cells, NK cells, and monocyte granulocytes.

• Comprehensive lineage panels, hematopoietic stem/progenitor cells, myeloid panels

• BAF immune profiling

• T cell subsets (Th1, Th2, Treg, activation, memory, exhaustion).

  Outputs

  % of populations, median fluorescence intensity, co-expression patterns.

• Live/dead discrimination using Zombie dyes, PI, 7-AAD and DAPI.

• Apoptosis/necrosis assays with Annexin V + PI

• Mitochondrial health assays (JC-1, TMRE/TMRM),

• ROS detection (DCFDA, CellROX)

• Cell cycle analysis DNA content (PI, DAPI, Hoechst)

  Outputs

  % viable/apoptotic/necrotic cells, ROS levels, mitochondrial depolarization, G0/G1/S/G2/M phase fractions.

Functional Immune Assays

Cellular Therapeutics

• Intracellular cytokine production (IFNγ, IL-4, TNFα, IL-17),

• Degranulation CD107a mobilization,

• phagocytosis assays, FITC-labeled bacteria/beads uptake

• oxidative burst assays Dihydrorhodamine, luminol-based.

• proliferation tracking via CFSE, CellTrace Violet, and eFluor dyes.

  Outputs

  % cytokine-positive cells, MFI shifts, proliferation index, phagocytic activity.

• CAR-T characterization including CAR expression and activation markers

• CRISPR/transfection GFP efficiency reporting

• Drug cytotoxicity monitoring

• Cell line authentication, surface marker profiling, and clonality confirmation.

  Outputs

  % transduced cells, dose-response curves, biomarker validation.

/ Screening Solutions

Specialized Screening Applications

Specialized Applications

Microbiology & Host-Pathogen

Extracellular Vesicles

96- or 384-well plate–assays:

• Drug cytotoxicity screens.

• Immunomodulator assays (checkpoint inhibitors, cytokine inducers).

• Small molecule or siRNA screens.

Kinetics & dose-response

In multi-well format.

Outputs

Plate heatmaps, IC50/EC50 calculations, multiparametric readouts.

Bacterial counts & viability

SYTO dyes, PI.

Fungal cell profiling

Yeast budding, hyphal growth.

Viral infection assays

Reporter gene expression, antigen staining.

Host–pathogen interaction

Bacteria/parasite uptake by immune cells.

Outputs

% infected cells, pathogen load, immune response markers.

Exosome/microvesicle detection Size <1 µm, fluorescence labeling.

Bead-based capture assays

EV surface proteins, multiplex exosome markers.

Outputs

EV concentration (events/µL), marker positivity, relative distribution.

Connect with our scientific team to design custom flow cytometry panels, high-throughput screens, or translational biomarker assays tailored to your discovery pipeline.